DCK Antibody (C-term)

SKU: orb1929569

Description

DCK Antibody (C-term)

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, IP, WB
• Dilution range: IHC-P - 1:100-500, IF - 1:10-50, WB - 1:1000, IP - 1:500-1000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 30519 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Confocal immunofluorescent analysis of DCK Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

DCK Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DCK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

The anti-DCK Pab is used in Western blot to detect DCK in mouse intestine tissue lysate.

Deoxycytidine kinase (DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody using the Pierce classic mammalian IP kit reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1: 700 dilution of DCK antibody. Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample.

Quick Database Links

UniProt

P27707