DCK Rabbit Polyclonal Antibody

SKU: orb402255

Description

The DCK Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, IHC, and WB applications. This antibody targets the Deoxycytidine kinase in Human, Mouse, and Rat samples.

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml Flow Cytometry(Fixed), 1-3μg/1x10^6 cells ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human DCK recombinant protein (Position: E17-L260).
• Target: Deoxycytidine kinase
• Molecular Weight: 30 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Deoxycytidine kinase; dCK; DCK

Quick Database Links

Gene Symbol

Deoxycytidine kinase

UniProt

P27707

Fei Feng, Bin Wang, Xiuxuan Sun, Yumeng Zhu, Hao Tang, Gang Nan, Lijuan Wang, Bo Wu, Muren Huhe, Shuangshuang Liu, Tengyue Diao, Rong Hou, Yang Zhang, Zheng Zhang Metuzumab enhanced chemosensitivity and apoptosis in non-small cell lung carcinoma Cancer Biology & Therapy, 18, 51 (2017)

PubMed: 28055291

Applications

IHC, WB

Reactivity

Human