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Rabbit Cul4B Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 863 and C-terminus |
| Target | Cul4B |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
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Detection of human Cul4B by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-Cul4B antibody orb1524037 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse Cul4B by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Cul4B (Cat. No. orb1524037) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Cul4B by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Cul4B (Cat. No. orb1524037) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003579.3 |
|---|
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Protocol Information
Rabbit Cul4B Antibody (orb1524037)
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