CUL4B Antibody (Center)

SKU: orb1931816

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2000
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD64743
• Immunogen: This CUL4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the Central region of human CUL4B. Antigen Region: 250-278 aa.
• Target: CUL4B {ECO:0000303|PubMed:14578910, ECO:0000312|HGNC:HGNC:2555}
• Molecular Weight: 103982 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cullin-4B, CUL-4B, CUL4B, KIAA0695

Western blot analysis of CUL4B Antibody (Center) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in Hela cell line lysate. CUL4B Antibody (Center) (arrow) was detected using the purified Pab.

Anti-CUL4B Antibody (Center) at 1:2000 dilution + human testis lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CUL4B Antibody (Center) at 1:250 dilution + MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 103 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CUL4B {ECO:0000303|PubMed:14578910, ECO:0000312|HGNC:HGNC:2555}

UniProt

Q13620

RefSeq (Protein):NP_003579.3, NP_001073341.1