CUL4B Antibody (Center)

• Catalog Number: orb1931816
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB42521
• Tested applications: WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:250, WB: 1:1000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 103982 Da
• Target: This CUL4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the Central region of human CUL4B.
• UniProt ID: Q13620
• NCBI: NP_003579.3, NP_001073341.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cullin-4B, CUL-4B, CUL4B, KIAA0695
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CUL4B Antibody (Center) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in Hela cell line lysate. CUL4B Antibody (Center) (arrow) was detected using the purified Pab.

Anti-CUL4B Antibody (Center) at 1:2000 dilution + human testis lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CUL4B Antibody (Center) at 1:250 dilution + MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 103 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.