Rabbit anti-CDK4 Antibody

• Catalog Number: orb1523392
• Category: Antibodies
• Description: CDK4 Antibody
• Target: CDK4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 253 and C-terminus
• UniProt ID: P11802
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: cell division protein kinase 4; CMM3; cyclin-depen
• Note: For research use only
• NCBI: NP_000066.1

Detection of human CDK4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat, cells. Antibodies: Affinity purified rabbit anti-CDK4 antibody orb1523392 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CDK4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CDK4 antibody orb1523392 used for IP at 6 µg/mg lysate.

Detection of human CDK4 by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CDK4 (Cat. No. orb1523392) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human CDK4 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CDK4 (Cat. No. orb1523392) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB