[KO Validated] CDKN2A/p16INK4a Rabbit pAb Discontinued

SKU: orb129709

Description

Rabbit polyclonal antibody against CDKN2A

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-156 of human CDKN2A/p16INK4a (NP_000068.1).
• Molecular Weight: 16kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-ARF antibody, anti-CCM2 antibody, anti-CDK4 inhibitor p16 INK4 antibody, anti-CDK4I antibody, anti-CDKN2 antibody, anti-CDKN2A antibody, anti-Cell cycle negative regulator beta antibody, anti-CMM2 antibody, anti-Cyclin dependent kinase inhibitor p16 antibody, anti-INK4 antibody, anti-INK4A antibody, anti-MLM antibody, anti-MTS1 antibody, anti-Multiple tumor suppressor 1 antibody, anti-p14 antibody, anti-p14ARF antibody, anti-p16 antibody, anti-P16INK4 antibody, anti-p16INK4a antibody, anti-p19 antibody, anti-p19Arf antibody, anti-TP16 antibody

Immunofluorescence analysis of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (orb129709) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of lysates from HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (orb129709) at 1:18000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Western blot analysis of lysates from HeLa cells, using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (orb129709) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of lysates from wild type(WT) and CDKN2A/p16INK4a knockout (KO) 293T(KO) cells, using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (orb129709) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Quick Database Links

UniProt

Q8N726, P42771

Kimoto, Saki et al. Nacre extract derived from Pinctada fucata mitigates skeletal muscle aging by suppressing senescence-associated inflammation and preserving contractile features in naturally aged mice and C2C12 myotubes Asian Pacific Journal of Tropical Biomedicine, (2026)

PubMed:

Applications

IF

Reactivity

Mouse