Rabbit Bcl-2 Antibody

SKU: orb1524399

Description

Bcl-2 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 3

Tested Applications	FC, IHC, IP
Dilution Range	WB - Not recommended. orb1524399 has not performed satisfactorily when used for WB of Bcl-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Bcl-2.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 50 and 100
Target	Bcl-2
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; PPP1R50; protein phosphatase 1, regulatory subunit 50

Bax Rabbit Polyclonal Antibody [orb4655]

Applications:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Porcine, Rabbit, Sheep
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 11
BCL2 Rabbit Polyclonal Antibody [orb10173]
Applications:
ELISA, ICC, IF, IHC-P, WB
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg
Item 1 of 6
Bcl-2 Rabbit Polyclonal Antibody [orb100697]
Applications:
FC, ICC
Predicted Reactivity:
Rat
Reactivity:
Human, Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl, 200 μl
Item 1 of 12
NCBP1 Rabbit Polyclonal Antibody [orb1743898]
Applications:
ELISA, FC, ICC, IF, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg

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Detection of human Bcl-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl-2 antibody orb1524399 used for IP at 6 µg/mg lysate.

Detection of human Bcl-2 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Bcl-2 (Cat. No. orb1524399) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Bcl-2 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bcl-2 (Cat. No. orb1524399) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

Bcl-2

UniProt

P10415

RefSeq (Protein):NP_000648.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000648.2

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Protocol Information

IHC

Immunohistochemistry

Flow Cytometry

Immunoprecipitation