BCL2 antibody

• Catalog Number: orb10173
• Category: Antibodies
• Description: BCL2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BCL2. Please contact us for the exact immunogen sequence. The peptide is available as orb374703.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: WB: 1:100-1000, IHC-P: 1:100-500, IF/ICC: 1:100-600 (based on 0.5mg/ml)
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 26 kDa
• Target: BCL2
• Entrez: 596
• UniProt ID: P10415, P49950, P10417
• NCBI: 000624, 21, 000633
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: anti-Apoptosis regulator Bcl 2 antibody, anti- Apo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Juanjuan Xiong, Zixu Wang, Yulan Dong, Jing Cao, Yaoxing Chen The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymus Poult Sci, 103331 (2023)

PubMed: 38100948

Applications

WB

Reactivity

Gallus

Yang, Xifeng et al. Downregulation of miR‑181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‑κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020)

PubMed: 32901872

Applications

WB

Reactivity

Human

Babaeenezhad, Esmaeel et al. D-Limonene Alleviates Acute Kidney Injury Following Gentamicin Administration in Rats: Role of NF-κB Pathway, Mitochondrial Apoptosis, Oxidative Stress, and PCNA Oxid Med Cell Longev, 2021, 6670007 (2021)

PubMed: 33510839

Applications

IHC

Reactivity

Rat

Immunohistochemical staining of human breast cancer tissue using BCL2 antibody (2.5 ug/ml)

WB analysis of rat lymph node (lane 1), mouse heart (lane 2), rat spleen (lane 3), rat thymus (lane 4), mouse pancreas (lane 5), human breast cancer (lane 6) using BCL2 antibody (1 ug/ml)

IHC-P staining of mouse lymph node tissue using anti-BCL2 (2.5 ug/ml)

IF image of mouse lymph node tissue using anti-BCL2 (2.5 ug/ml)

Western blot analysis of rat lymph node (lane 1), mouse heart (lane 2), rat spleen (lane 3), rat thymus (lane 4), mouse pancreas (lane 5), human breast cancer (lane 6) using BCL2 antibody (1 ug/ml)

Immunohistochemical staining of mouse lymph node tissue using anti-BCL2 (2.5 ug/ml)

IHC-P staining of human breast cancer tissue using anti-BCL2 (2.5 ug/ml)

Immunofluorescence image of mouse lymph node tissue using BCL2 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded human breast cancer tissue using anti-BCL2 (2.5 ug/ml)

IHC-P image of human breast cancer tissue using BCL2 antibody (2.5 ug/ml)

IHC-P staining of human breast cancer tissue using BCL2 antibody (2.5 ug/ml)