BCL2 antibody

SKU: orb10173

Description

BCL2 antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Dilution range: WB: 1:100-1000, IHC-P: 1:100-500, IF/ICC: 1:100-600 (based on 0.5mg/ml)

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BCL2. Please contact us for the exact immunogen sequence. The peptide is available as orb374703.
• Target: BCL2
• Molecular Weight: 26 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti-Apoptosis regulator Bcl 2 antibody, anti- Apoptosis regulator BCL2 antibody, anti- Apoptosis regulator BCL2 antibody, anti- AW986256 antibody, anti- B cell CLL/lymphoma 2 antibody, anti- B cell leukemia/lymphoma 2 antibody, anti- BCL2 antibody, anti- BCL2 antibody, anti- BCL2_human antibody, anti- C430015F12Rik antibody, anti- D630044D05Rik antibody, anti- D830018M01Rik antibody, anti- Leukemia/lymphoma, B-cell, 2 antibody, anti- Oncogene B-cell leukemia 2 antibody, anti- PPP1R50 antibody, anti- Protein phosphatase 1, regulatory subunit 50 antibody

Immunohistochemical staining of human breast cancer tissue using BCL2 antibody (2.5 ug/ml)

WB analysis of rat lymph node (lane 1), mouse heart (lane 2), rat spleen (lane 3), rat thymus (lane 4), mouse pancreas (lane 5), human breast cancer (lane 6) using BCL2 antibody (1 ug/ml)

IHC-P staining of mouse lymph node tissue using anti-BCL2 (2.5 ug/ml)

IF image of mouse lymph node tissue using anti-BCL2 (2.5 ug/ml)

Western blot analysis of rat lymph node (lane 1), mouse heart (lane 2), rat spleen (lane 3), rat thymus (lane 4), mouse pancreas (lane 5), human breast cancer (lane 6) using BCL2 antibody (1 ug/ml)

Immunohistochemical staining of mouse lymph node tissue using anti-BCL2 (2.5 ug/ml)

IHC-P staining of human breast cancer tissue using anti-BCL2 (2.5 ug/ml)

Immunofluorescence image of mouse lymph node tissue using BCL2 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded human breast cancer tissue using anti-BCL2 (2.5 ug/ml)

IHC-P image of human breast cancer tissue using BCL2 antibody (2.5 ug/ml)

IHC-P staining of human breast cancer tissue using BCL2 antibody (2.5 ug/ml)

Quick Database Links

Gene Symbol

BCL2

UniProt

P49950, P10415, P10417

NCBI Gene ID

596

RefSeq:000633, 000624, 21

References

Babaeenezhad, Esmaeel et al. D-Limonene Alleviates Acute Kidney Injury Following Gentamicin Administration in Rats: Role of NF-κB Pathway, Mitochondrial Apoptosis, Oxidative Stress, and PCNA Oxid Med Cell Longev, 2021, 6670007 (2021)

PubMed: 33510839

Applications

IHC

Reactivity

Rat

Juanjuan Xiong, Zixu Wang, Yulan Dong, Jing Cao, Yaoxing Chen The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymus Poult Sci, 103331 (2023)

PubMed: 38100948

Applications

WB

Reactivity

Gallus

Amir Mohammad Zobeydi 1, Seyede Nasim Mousavi Namavar 2, Maryam Sadeghi Shahdani 2, Siroos Choobineh 3, Mohammad Reza Kordi 2, Kamran Rakhshan 4 Mitigating doxorubicin-induced hepatotoxicity in male rats: The role of aerobic interval training and curcumin supplementation in reducing oxidative stress, endoplasmic reticulum stress and apoptosis Sci Rep, (2025)

PubMed: 39994295

Applications

WB

Reactivity

Rat

Qiang Wang 1, Ying Zhang 1, Yuanyuan Zhang 1, Yuanyuan Zhai 1, Xinyao Zhou 1, Yun Qin 2, Ming Ding 2, Yuan Tian 1, Zunjian Zhang 1, Pei Zhang 3, Fengguo Xu Triflupromazine and tranylcypromine alleviate primary cisplatin resistance in lung adenocarcinoma by promoting LDHA-mediated AMBRA1 ubiquitination Biochem Pharmacol ., (2025)

PubMed: 40562117

Yang, Xifeng et al. Downregulation of miR‑181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‑κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020)

PubMed: 32901872

Applications

WB

Reactivity

Human