Bcl-2 Rabbit Polyclonal Antibody

• Catalog Number: orb100697
• Category: Antibodies
• Description: Bcl-2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Bcl-2 (161-239/239aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 26 kDa
• Target: BCL2
• UniProt ID: P10415
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Apoptosis regulator Bcl 2; Apoptosis regulator Bcl
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Mantawy, Eman M. et al. Chrysin alleviates acute doxorubicin cardiotoxicity in rats via suppression of oxidative stress, inflammation and apoptosis Eur J Pharmacol, 728, 107-118 (2014)

PubMed: 24509133

Applications

IHC

Reactivity

Rat

Haorong He 1 2, Yuanhang Wei 1 2, Yuqi Chen 1 2, Xiyu Zhao 1 2, Xiaoxu Shen 1 2, Qing Zhu 3 4, Huadong Yin High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein J Anim Sci Biotechnol ., 15, 42 (2024)

PubMed: 38468340

Applications

IHC

Reactivity

Gallus

Tomar, Ameesha et al. The dietary isoflavone daidzein mitigates oxidative stress, apoptosis, and inflammation in CDDP-induced kidney injury in rats: Impact of the MAPK signaling pathway J Biochem Mol Toxicol, 34, e22431 (2020)

PubMed: 31833131

Applications

IHC

Reactivity

Rat

Mahdy, Heba M. et al. The anti-apoptotic and anti-inflammatory properties of puerarin attenuate 3-nitropropionic-acid induced neurotoxicity in rats Can J Physiol Pharmacol, 92, 252-258 (2014)

PubMed: 24593790

Reactivity

Rat

Blank control: mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA (green).

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (orb100697) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (orb100697) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (orb100697) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (orb100697) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868589) for 90 minutes, and DAPI for nuclei staining.

Tissue/cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Bcl-2 Polyclonal Antibody, Unconjugated (orb100697) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.