Rabbit 4EBP1 Antibody

SKU: orb1530099

Description

4EBP1 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB - 1:1,000 - 1:6,000; IHC - 1:5,000 - 1:20,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 68 and C-terminus
• Target: 4EBP1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4EBP1; 4E-BP1; BP-1; eIF4E-binding protein 1; eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; phosphorylated heat- and acid-stable protein regulated by insulin 1

Detection of 4EBP1 by western blot.Samples: Whole cell lysate from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody: Affinity purified rabbit anti-4EBP1 (orb1530099) used at 0.25 µg/ml.

Detection of human 4EBP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20, 000 (0.05µg/ml).

Quick Database Links

Gene Symbol

4EBP1

UniProt

Q13541

RefSeq (Protein):NP_004086.1