Phospho-eIF4EBP1 (Thr70) Rabbit Polyclonal Antibody

SKU: orb183352

Description

Phospho-eIF4EBP1 (Thr70) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Mouse, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Thr70 TK(p-T)P
• Target: EIF4EBP1
• Molecular Weight: 12 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

eIF4EBP1(phospho T70); eIF4EBP1(phospho Thr70); p-eIF4EBP1(Thr70); Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; phosphorylated heat-and acid-stable protein regulated by insulin 1(PHAS-I); 4EBP1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (phospho-eIF4EBP1 (Thr70)) Polyclonal Antibody, Unconjugated (orb183352) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Ovary (Rat) Tissue Lysate at 30 ug, Primary: Anti-phospho-eIF4EBP1 (Thr70) (orb183352) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12kD, Observed band size: 12kD.

Quick Database Links

Gene Symbol

EIF4EBP1

UniProt

Q13541