eIF4EBP1 Rabbit mAb [KD Validated]

SKU: orb1951291

Description

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution range: WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human eIF4EBP1
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Disclaimer: For research use only

Alternative Names

4E-BP1; 4EBP1; BP-1; MGC4316; PHAS-I;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of HeLa cells, using eIF4EBP1 Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using eIF4EBP1 Antibody.

Western blot analysis of eIF4EBP1 expression in K562 cell lysate.

Quick Database Links

UniProt

Q13541