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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | ICC, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 |
| Reactivity | Human, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary. |
| Target | Pyruvate Kinase (liver/RBC) |
| Molecular Weight | 62 |
| Purity | Purified by antigen-affinity chromatography. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Buffer/Preservatives | 0.1M Tris, 0.1M Glycine, 10% Glycerol, 0.01% Thimerosal. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−pyruvate kinase L/R , PK1 , PKL , PKRL , RPK
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Immunofluorescence analysis of HeLa cells using Pyruvate Kinase (liver/RBC) antibody
Immunohistochemical staining of rat liver using Pyruvate Kinase (liver/RBC) antibody
Immunohistochemical staining of NCIN87 xenograft using Pyruvate Kinase (liver/RBC) antibody
Western blot analysis of 293T(Lane 1), A431(Lane 2), HeLa(Lane 3), HepG2(Lane 4), A375(Lane 5) using Pyruvate Kinase (liver/RBC) antibody
Western blot analysis of 293T(Lane 1), A431(Lane 2), HeLa(Lane 3), HepG2(Lane 4), A375(Lane 5) using Pyruvate Kinase (liver/RBC) antibody
Quick Database Links
Gene Symbol
Pyruvate Kinase (liver/RBC)
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ICC
Immunocytochemistry
Pyruvate Kinase Rabbit Polyclonal Antibody (orb556755)
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