PDK4 Antibody (C-term)

SKU: orb1929617

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:100, WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD25263
• Immunogen: This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4. Antigen Region: 382-410 aa.
• Target: PDK4
• Molecular Weight: 46469 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4

Fluorescent confocal image of HeLa cells stained with PDK4 (C-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PDK4 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Staining PDK4 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-PDK4 Antibody (C-term) at 1:2000 dilution + human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PDK4 Antibody (C-term) at 1:2000 dilution. Lane 1: human heart lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PDK4 Antibody (C396) at 1:2000 dilution. Lane 1: human heart lysates. Lane 2: mouse skeletal muscle lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PDK4

UniProt

Q16654

RefSeq (Protein):NP_002603.1