PDK4 Antibody (C-term)

• Catalog Number: orb1929617
• Category: Antibodies
• Description: PDK4 Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q16654
• MW: 46469 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PDHK4
• Research Area: Cell Biology, Metabolism Research, Signal Transduc
• Note: For research use only

Fluorescent confocal image of HeLa cells stained with PDK4 (C-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PDK4 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Staining PDK4 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-PDK4 Antibody (C-term) at 1:2000 dilution + human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PDK4 Antibody (C-term) at 1:2000 dilution. Lane 1: human heart lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PDK4 Antibody (C396) at 1:2000 dilution. Lane 1: human heart lysates. Lane 2: mouse skeletal muscle lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.