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Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

Catalog Number: orb5565

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb5565
CategoryAntibodies
DescriptionPhospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody
TargetPKM
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Rabbit, Rat
Predicted ReactivityBovine, Canine, Equine, Guinea pig, Mouse, Porcine
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human PKM2 around the phosphorylation site of Tyr105 IL(p-Y)RP
UniProt IDP14618
RRIDAB_10929812
MW58 kDa
Tested applicationsELISA, FC, IF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesPKM2(phospho Tyr105); PKM2(phospho Y105); PK 1; PK
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NoteFor research use only
Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

Blank control: A549. Primary Antibody (green line): Rabbit Anti-Phospho-PKM2 (Tyr105) antibody (orb5565), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

phosphopeptide, non phosphopeptide.

Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

Tissue/Cell: human endometrium tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

Tissue/Cell: human liver carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-PKM2 Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

Tissue/Cell: Rat Thyroid tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-NMDAR1 (Ser890) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4°C, followed by conjugati Phospho-PKM2on to the secondary antibody and DAB staining.

Phospho-PKM2 (Tyr105) Rabbit Polyclonal Antibody

Tissue/Cell: rat thyroid tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (orb5565) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.