Pyridostatin (RR82)

SKU: orb1220174

Description

Pyridostatin (RR82) hydrochloride (10 μM; 48 hours) induces cell cycle arrest.Pyridostatin hydrochloride is a very selective G-quadruplex DNA-binding small molecule designed to form a complex with and stabilize G-quadruplex structure. Pyridostatin hydrochloride causes neurite retraction, synaptic loss, and dose-dependent neuronal death. In cultured primary neurons, Pyridostatin hydrochloride induces the formation of DNA DSBs. Remarkably, Pyridostatin hydrochloride (1-5 μM, overnight) downregulates the BRCA1 protein, a protein that guards and repairs the neuronal genome, at the transcriptional level.

Key Properties

• CAS Number: 1781882-65-2
• MW: 778.94
• Purity: >98% (HPLC)
• Formula: C??H??Cl?N?O?
• SMILES: O=C(C1=CC(OCCN)=CC(C(NC2=NC3=C(C(OCCN)=C2)C=CC=C3)=O)=N1)NC4=CC(OCCN)=C5C=CC=CC5=N4.Cl.Cl.Cl.Cl.Cl
• Solubility: H2O : 50 mg/mL (64.19 mM; Need ultrasonic)

Bioactivity

• In Vitro:
  Pyridostatin (RR82) hydrochloride (10 μM; 48 hours) induces cell cycle arrest. Pyridostatin hydrochloride is a very selective G-quadruplex DNA-binding small molecule designed to form a complex with and stabilize G-quadruplex structure. Pyridostatin hydrochloride causes neurite retraction, synaptic loss, and dose-dependent neuronal death. In cultured primary neurons, Pyridostatin hydrochloride induces the formation of DNA DSBs. Remarkably, Pyridostatin hydrochloride (1-5 μM, overnight) downregulates the BRCA1 protein, a protein that guards and repairs the neuronal genome, at the transcriptional level. Cell Viability Assay Cell line: Over 60 different cancer cell lines. Concentration: 10 μM. Incubation time: 48 hours. Result: Predominantly accumulated in the G2 phase of the cell cycle over 60 different cancer cell lines.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RR82 hydrochloride