Pyridostatin TFA

SKU: orb1302832

Description

Pyridostatin trifluoroacetate is a potent G-quadruplex stabilizer (Kd = 490 nM) targeting proto-oncogenes such as c-kit, K-ras, and Bcl-2. It is widely used in biochemical and cellular assays to study gene expression regulation and has been applied in both in vitro and in vivo cancer research models.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 1472611-44-1
• MW: 938.71
• Purity: 99.84% (May vary between batches)
• Formula: C₃₇H₃₅F₉N₈O₁₁
• SMILES: OC(=O)C(F)(F)F.NCCOc1cc(nc(c1)C(=O)Nc1cc(OCCN)c2ccccc2n1)C(=O)Nc1cc(OCCN)c2ccccc2n1
• Target: DNA/RNA Synthesis
• Solubility: DMSO:52 mg/mL (55.4 mM)

Bioactivity

• Target IC50:
  G-quadruplexe (Cell-free assay):490 nM(Kd)
• In Vitro:
  Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC.Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes.
• Cell Research:
  Cell lines: MRC-5–SV40 cells and various cancer cell linesConcentrations: 10 μMIncubation Time: 48 hMethod: Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

arrest, cycle, damage, DSB, DNASynthesis, DNA, DNA Synthesis, G-quadruplex, Inhibitor, neurodegeneration, inhibit, Pyridostatin, Pyridostatin TFA, structure, SRC, RR 82, RR82, RR-82, RNA Synthesis, RNASynthesis

Compound Identifiers

PubChem CID

117064504