Pyridostatin Trihydrochloride

SKU: orb1302844

Description

Pyridostatin Trihydrochloride (RR-82 Trihydrochloride) is a potent small molecule that selectively stabilizes G-quadruplex DNA structures with a dissociation constant (Kd) of 490 nM. It is widely used as a chemical probe in vitro to study telomere maintenance and gene regulation, and has been employed in vivo to investigate its potential in oncology research.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 2517456-88-9
• MW: 706.02
• Purity: 99.69% (May vary between batches)
• Formula: C₃₁H₃₅Cl₃N₈O₅
• SMILES: Cl.Cl.Cl.NCCOc1cc(nc(c1)C(=O)Nc1cc(OCCN)c2ccccc2n1)C(=O)Nc1cc(OCCN)c2ccccc2n1
• Target: DNA/RNA Synthesis
• Solubility: DMSO:50 mg/mL (70.82 mM);H2O:7.09 mg/mL (10.04 mM)

Bioactivity

• Target IC50:
  G-quadruplexe:Kd:490 nM
• In Vitro:
  Pyridostatin hydrochloride is a G-quadruplexe stabilizer, with a Kd of 490 nM. Pyridostatin (PDS) shows neurotoxic activity against primary cortical neurons at 0.01-5 μM, causes DNA double-strand breaks (DSBs) at 1 μM, downregulates BRCA1 in neurons at 1, 2 or 5 μM. Pyridostatin interacts with G-quadruplex motifs in SRC and alters mRNA levels of damaged genes
• Cell Research:
  Cells are plated at equal confluence and either untreated or treated with 2 μM Pyridostatin continually for 72 h. Cells from individual plates are trypsinized and counted in a Coultercounter. Graphs represent total cell numbers at each time interval and error bars represent S.E.M. Data represent three independent experiments.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA Synthesis, DNASynthesis, G-quadruplex, Pyridostatin, Pyridostatin Trihydrochloride(free base 1085412-37-8 ), Pyridostatin Trihydrochloride, RNA Synthesis, RNASynthesis, RR 82 Trihydrochloride, RR 82, RR82, RR-82, RR82 Trihydrochloride, RR-82 Trihydrochloride

Compound Identifiers

PubChem CID

137891317