PUF60 Antibody

• Catalog Number: orb1256829
• Category: Antibodies
• Description: PUF60 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 65kDa
• Target: PUF60
• UniProt ID: Q9UHX1
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PUF60, FIR, FLJ31379, RoBPI, SIAHBP1, VRJS
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PUF60 antibody (orb1256829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PUF60 antibody (orb1256829) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using PUF60 antibody (orb1256829). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 150 ug extracts of A549 cells using 3 ug PUF60 antibody (orb1256829). Western blot was performed from the immunoprecipitate using PUF60 antibody (orb1256829) at a dilution of 1:500.