SIAHBP1/FIR Antibody

SKU: orb18885

Description

Goat polyclonal antibody to PUF60

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in lysate of human epidermoid carcinoma A431 (calculated MW of 59.9kDa (isoform A) according to NP_510965.1 and 58.2kDa (isoform B) according to NP_055096.2). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SIAHBP1 / FIR
• Protein Sequence: YDQERFDNSDLSA
• Molecular Weight: 59.9; 58.2; 55.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PUF60 antibody, anti SIAHBP1 antibody, anti FIR antibody, anti fuse-binding protein-interacting repressor antibody, anti RoBPI antibody, anti siah binding protein 1 antibody, anti poly-U binding splicing factor PUF60 antibody, anti Ro ribonucleoprotein-binding protein 1 antibody, anti pyrimidine tract binding splicing factor antibody, anti poly-U binding splicing factor 60KDa antibody, anti FLJ31379 antibody, anti FBP interacting repressor antibody, anti Ro ribonucleoprotein binding protein 1 antibody

Western blot analysis of A431 lysate

Western blot analysis of HEK293 lysate using PUF60 antibody

Immunohistochemical staining of Human Prostate using PUF60 antibody

Immunohistochemical staining of Human Skin using PUF60 antibody

Quick Database Links

Gene Symbol

SIAHBP1 / FIR

NCBI Gene ID

22827