PRDM1 Antibody

• Catalog Number: orb1248924
• Category: Antibodies
• Description: PRDM1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DISDNADRLEDVED
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 90kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100kDa in lysates of cell line K562. An additional faint band of approx 70kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8kDa according to NP_001189.2 isoform 1 and 76.8kD according to NP_878911.1 isoform 2). Recommended concentration: 0.1-2g/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PRDM1
• UniProt ID: O75626
• NCBI: NP_878911.1, NP_001189.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PRDM1, PR domain containing 1, with ZNF domain, BL
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 90kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100kDa in lysates of cell line K562. An additional faint band of approx 70kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8kDa according to NP_001189.2 isoform 1 and 76.8kD according to NP_878911.1 isoform 2). Recommended concentration: 0.1-2g/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248924 (3.8 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1248924 (0.1 ug/ml) staining of A431 (A), (2 ug/ml) K562 (B) and (1 ug/ml) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248924 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining.

orb1248924 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).