PRDM1/BLIMP1 Antibody

SKU: orb18713

Description

Goat polyclonal to PRDM1

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: ELISA, IF, IHC
• Dilution Range: ELISA: 1:32000, IHC-P: 10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  This antibody is expected to recognize both reported isoforms (NP_001189.2 and NP_878911.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 100kDa band observed in Human Brain (cerebellum) lysates (calculated MW of 91.8kDa according to NP_001189.2 and of 76.8 according to NP_878911.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PRDM1 / BLIMP1
• Protein Sequence: KVKQETVEPMDP
• Molecular Weight: 91.8; 76.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PRDM1 antibody, anti PR domain containing 1, with ZNF domain antibody, anti RP1-134E15.1 antibody, anti BLIMP1 antibody, anti MGC118922 antibody, anti MGC118923 antibody, anti MGC118924 antibody, anti MGC118925 antibody, anti PRDI-BF1 antibody, anti B-lymphocyte-induced maturation protein 1 antibody, anti PR domain containing 1, with ZNF domain, isoform 2 antibody, anti PR-domain zinc finger protein 1 antibody, anti PRDI-binding factor-1 antibody, anti beta-interferon gene positive-regulatory domain I binding factor antibody, anti positive regulatory domain I-binding factor 1 antibody

Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

6 μg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.

Quick Database Links

Gene Symbol

PRDM1 / BLIMP1

NCBI Gene ID

639