Goat anti-PRDM1 / BLIMP1 Antibody

• Catalog Number: orb18713
• Category: Antibodies
• Description: Goat polyclonal to PRDM1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:32000, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 91.8; 76.8
• Target: PRDM1 / BLIMP1
• Entrez: 639
• Protein Sequence: KVKQETVEPMDP
• RRID: AB_10748633
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PRDM1 antibody, anti PR domain containing 1,
• Note: For research use only
• Application notes: This antibody is expected to recognize both reported isoforms (NP_001189.2 and NP_878911.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 100kDa band observed in Human Brain (cerebellum) lysates (calculated MW of 91.8kDa according to NP_001189.2 and of 76.8 according to NP_878911.1). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

6 μg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.