POU2AF1 Antibody

• Catalog Number: orb1247918
• Category: Antibodies
• Description: POU2AF1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-DAYALNHTLSVEGF
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 30kDa band seen in mouse spleen lysate (Predicted MW of 28kDa according to NP_006226). Recommended for use at 1-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: POU2AF1
• UniProt ID: Q16633
• NCBI: NP_006226.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: BOB1, OBF1, POU2AF1, OBF-1, POU domain, class 2, a
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 30kDa band seen in mouse spleen lysate (Predicted MW of 28kDa according to NP_006226). Recommended for use at 1-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247918 (0.3 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human BOB1 with DYKDDDDK tag probed with orb1247918 (1 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with orb1247918 (1 mg/ml) in Lane B.