POU2AF1 Antibody

SKU: orb1247918

Description

POU2AF1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 30kDa band seen in mouse spleen lysate (Predicted MW of 28kDa according to NP_006226). Recommended for use at 1-3µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DAYALNHTLSVEGF
• Target: POU2AF1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BOB1, OBF1, POU2AF1, OBF-1, POU domain, class 2, associating factor 1, POU class 2 associating factor 1, OCAB

orb1247918 (0.3 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human BOB1 with DYKDDDDK tag probed with orb1247918 (1 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with orb1247918 (1 mg/ml) in Lane B.

Quick Database Links

Gene Symbol

POU2AF1

UniProt

Q16633

RefSeq (Protein):NP_006226.2