POU2AF1 Antibody

SKU: orb22487

Description

Goat polyclonal antibody to POU2AF1

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  WB: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.5-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: POU2AF1
• Molecular Weight: 27.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti BOB1 antibody, anti OBF1 antibody, anti OBF-1 antibody, anti OCAB antibody, anti POU class 2 associating factor 1 antibody, anti POU domain, class 2, associating factor 1 antibody, anti POU2AF1 antibody

Western blot analysis of HEK293 lysate using POU2AF1 antibody

Western blot analysis of Daudi lysate using POU2AF1 antibody

Quick Database Links

Gene Symbol

POU2AF1

NCBI Gene ID

5450