POU2AF1 Antibody

SKU: orb22486

Description

Goat polyclonal antibody to POU2AF1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 1-2 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  IHC: In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5μg/ml.WB: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: POU2AF1
• Molecular Weight: 27.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti BOB1 antibody, anti OBF1 antibody, anti OBF-1 antibody, anti OCAB antibody, anti POU class 2 associating factor 1 antibody, anti POU domain, class 2, associating factor 1 antibody, anti POU2AF1 antibody

Western blot analysis of HEK293 lysate (10ug protein in RIPA buffer) DYKDDDDK tag in (Lane A), Mock-transfected HEK293 in (Lane B) and anti-DYKDDDDK Tag (1/3000) in (lane C) using POU2AF1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human Spleen shows nuclear tissue using POU2AF1 antibody

Quick Database Links

Gene Symbol

POU2AF1

NCBI Gene ID

5450