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Item 1 of 4
Item 1 of 4
POLRMT (CT) Antibody
Catalog Number: orb1672346
| Catalog Number | orb1672346 |
|---|---|
| Category | Antibodies |
| Description | POLRMT (CT) Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, WB |
| Predicted Reactivity | Bovine, Feline, Monkey, Primate |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Anti-POLRMT antibody (orb1672346) was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human POLRMT. The immunogen is located within the last 50 amino acids of POLRMT. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Predicted: 139kD Observed: 139 kD |
| Target | POLRMT |
| UniProt ID | O0411 |
| NCBI | O0411.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | POLRMT Antibody is supplied in PBS containing 0.02% sodium azide. |
| Alternative names | DNA-directed RNA polymerase, mitochondrial, MtRPOL Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
WB Validation in Human Cell Lines. Loading: 15 µg of lysate. Antibodies: POLRMT, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of POLRMT in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling POLRMT with orb1672346 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of POLRMT in Mouse Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling POLRMT with orb1672346 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 4 Immunofluorescence Validation of POLRMT in Rat Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling POLRMT with orb1672346 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
