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POLRMT Antibody
SKU: orb1672346
Description
Images & Validation
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| Tested Applications | ELISA, IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Feline, Monkey, Primate |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-POLRMT antibody (orb1672346) was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human POLRMT. The immunogen is located within the last 50 amino acids of POLRMT. |
| Target | POLRMT |
| Molecular Weight | Predicted: 139kD Observed: 139 kD |
| Purification | POLRMT Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | POLRMT Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−DNA-directed RNA polymerase, mitochondrial, MtRPOL, POLRMT Isoforms
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WB Validation in Human Cell Lines. Loading: 15 µg of lysate. Antibodies: POLRMT, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of POLRMT in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling POLRMT with orb1672346 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of POLRMT in Mouse Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling POLRMT with orb1672346 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 4 Immunofluorescence Validation of POLRMT in Rat Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling POLRMT with orb1672346 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
POLRMT Antibody (orb1672346)
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