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POLRMT Antibody
SKU: orb1259431
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3). |
| Target | POLRMT |
| Molecular Weight | Observed: 139kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−POLRMT, polymerase (RNA) mitochondrial (DNA directed), APOLMT, MTRNAP, MTRPOL, h-mtRPOL, DNA-directed RNA polymerase, mitochondrial
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Western blot analysis of extracts of various cell lines, using POLRMT antibody (orb1259431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation analysis of 200 ug extracts of HepG2 cells, using 3 ug POLRMT antibody (orb1259431). Western blot was performed from the immunoprecipitate using POLRMT antibody (orb1259431) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
POLRMT
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
POLRMT Antibody (orb1259431)
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