POLRMT Antibody

• Catalog Number: orb1259431
• Category: Antibodies
• Description: POLRMT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 139kDa
• Target: POLRMT
• UniProt ID: O00411
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: POLRMT, polymerase (RNA) mitochondrial (DNA direct
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using POLRMT antibody (orb1259431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation analysis of 200 ug extracts of HepG2 cells, using 3 ug POLRMT antibody (orb1259431). Western blot was performed from the immunoprecipitate using POLRMT antibody (orb1259431) at a dilution of 1:1000.