POLRMT Antibody

SKU: orb1259431

Description

POLRMT Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IP, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).
• Target: POLRMT
• Molecular Weight: Observed: 139kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

POLRMT, polymerase (RNA) mitochondrial (DNA directed), APOLMT, MTRNAP, MTRPOL, h-mtRPOL, DNA-directed RNA polymerase, mitochondrial

Western blot analysis of extracts of various cell lines, using POLRMT antibody (orb1259431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation analysis of 200 ug extracts of HepG2 cells, using 3 ug POLRMT antibody (orb1259431). Western blot was performed from the immunoprecipitate using POLRMT antibody (orb1259431) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

POLRMT

UniProt

O00411