PINK1 Antibody

• Catalog Number: orb150676
• Category: Antibodies
• Description: Mouse Anti-Human PINK1 Monoclonal IgG1
• Target: PINK1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
• Purification: Protein G Purified
• Immunogen: Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. 82% identical to rat and 81% identical to mouse. > 30% identity with DMPK.
• UniProt ID: Q9BXM7
• MW: 50kDa
• Tested applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000)
• Application notes: 1 µg/ml of SMC-450 was sufficient for detection of PINK1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: N4/15 (Formerly sold as S4-15)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Parkinson disease (autosomal recessive) 6 Antibody
• Research Area: Cardiovascular Research, Neuroscience, Protein Bio
• Note: For research use only
• Entrez: 65018
• NCBI: NP_115785.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PINK1 Monoclonal Antibody, Clone N4/15. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) PINK1 Antibody (D) Composite.