PINK1 Antibody

SKU: orb167066

Description

Mouse monoclonal antibody to PINK1.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:100-500, IHC-P: 1:50-100, IF/ICC: 1:25
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 38CT20.8.5
• Immunogen: Recombinant PINK1 protein was used to produced this monoclonal antibody. Antigen Region: Unknown.
• Target: PINK1
• Molecular Weight: 62769
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti PINK1 antibody, anti Serine/threonine-protein kinase PINK1, mitochondrial antibody, anti BRPK antibody, anti PTEN-induced putative kinase protein 1 antibody

All lanes: Anti-Pink1 (115-213) at 1:1000 dilution. Lane 1: HeLa whole cell lysate. Lane 2: MCF-7 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of PC12 cells stained with Pink1 (115-213) Antibody. diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. heart section using Pink1 (115-213). diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. stomach section using Pink1 (115-213). diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Quick Database Links

Gene Symbol

PINK1

UniProt

Q9BXM7

RefSeq (Protein):NP_115785.1

Ordureau, Alban et al. Dynamics of PARKIN-Dependent Mitochondrial Ubiquitylation in Induced Neurons and Model Systems Revealed by Digital Snapshot Proteomics Mol Cell, 70, 211-227.e8 (2018)

PubMed: 29656925