Phospho-MEK1 (Ser298) (H8) rabbit mAb

• Catalog Number: orb1946150
• Category: Antibodies
• Description: Phospho-MEK1 (Ser298) (H8) rabbit mAb
• Clonality: Monoclonal
• Clone Number: MEK1S298-H8
• Tested applications: FC, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser298 of human phospho MEK1.
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q02750
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Dual specificity mitogen-activated protein kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of C6 cell extract untreated or treated with TPA using 0.01 μg/mL Phospho-MEK1 (S298) antibody MEK1S298-H8. Cat. #orb1946150.

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with MG132 (green) or treated and blocked with phospho peptide (orange) or treated and blocked with non-phospho peptide (purple) using Phospho-MEK1 (S298) antibody MEK1S298-H8 at 0.1 μg/mL. Cat. #orb1946150.

Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or treated with imatinib (red) or treated with pervanadate (green) using Phospho-MEK1 (S298) antibody MEK1S298-H8 0.5 μg/mL. Cat. #orb1946150.

Western blot analysis of C6 cell extract untreated or treated with TPA using 5 ng/mL Phospho-MEK1 (Ser298) antibody MEK1S98-H8. Cat.#orb1946150 or Company C antibody at 104 ng/mL (manufacturer's recommended concentration) developed using the same exposure.