Phospho-MEK1/2 (Ser221) (D3) rabbit mAb

• Catalog Number: orb1946141
• Category: Antibodies
• Description: Phospho-MEK1/2 (Ser221) (D3) rabbit mAb
• Clonality: Monoclonal
• Clone Number: MEK12S221-D3
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser221 of human phospho MEK1/2.
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P36507, Q02750
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Dual specificity mitogen-activated protein kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HT1080 cell extract untreated or treated with TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser221) antibody MEK12S221-D3. Cat.#orb1946141.

Western blot analysis of C2C12 cell extract untreated or treated with TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser221) antibody MEK12S221-D3. Cat.#orb1946141.

Western blot analysis of NIH3T3 cell extract untreated or treated with TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser221) antibody MEK12S221-D3. Cat.#orb1946141.

Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate (green) or imatinib and blocked with phospho peptide (gold) or pervanadate and blocked with phospho peptide (black) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-MEK1/2 (S221) antibody MEK12S221-D3 at 0.1 μg/mL. Cat. #orb1946141.

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-MEK1/2 (S221) antibody MEK12S221-D3 0.1 μg/mL. Cat. #orb1946141.

Western blot analysis of A549 cell extract untreated or treated with TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser221) antibody MEK12S221-D3 Cat. #orb1946141 or Company A antibody at 0.005 μg/mL (manufacturer's recommended concentration).