Phospho-MEK1/2 (Ser217/221) (H2) rabbit mAb

• Catalog Number: orb1946115
• Category: Antibodies
• Description: Phospho-MEK1/2 (Ser217/221) (H2) rabbit mAb
• Clonality: Monoclonal
• Clone Number: MEK12S217S221-H2
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser217/221 of human phospho MEK1/2.
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P36507, Q02750
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Dual specificity mitogen-activated protein kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa cell extract untreated or treated with UV + TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser217/221) antibody MEK12S217S221-H2. Cat.#orb1946115.

Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or stained with isotype control #orb1946241 (orange) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho peptide (gold) or treated and blocked with phospho peptide (black) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-MEK1/2 (S217/221) antibody MEK12S217S221-H2 at 0.01 μg/mL. Cat. #orb1946115.

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-MEK1/2 (S217/221) antibody MEK12S217S221-H2 0.01 μg/mL. Cat. #orb1946115.

Western blot analysis of HeLa cell extract untreated or treated with UV + TPA using 0.1 μg/mL Phospho-MEK1/2 (Ser217/221) antibody MEK12S217S221-H2. Cat.#orb1946115 or Company C antibody at 0.4 μg/mL (manufacturer's recommended concentration) developed using the same exposure.