PECAM-1/CD31 Antibody

SKU: orb1535148

Description

PECAM-1/CD31 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC, IHC-P, WB
• Dilution Range: Flo (1:100), IF (1:100), IHC, IHC-P (20 µg/ml), WB (1:500 - 1:2000)
• Reactivity: Canine, Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 1H6
• Immunogen: Full length human recombinant protein of human PECAM1(NP_00433) produced in HEK293T cell.
• Target: PECAM-1 / CD31
• Purification: Immunoaffinity purified from tissue culture supernatant
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% rAlbumin
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA

Anti-PECAM-1 / CD31 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Anti-PECAM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1.

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-PECAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PECAM1 antibody, and then analyzed by flow cytometry.

Quick Database Links

Gene Symbol

PECAM-1 / CD31