CD31 antibody

SKU: orb10314

Description

CD31 antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Dilution range: WB:2 μg/ml, IHC-P:1:500, IF/ICC: 1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD31. Please contact us for the exact immunogen sequence. The peptide is available as orb374712.
• Target: CD31
• Molecular Weight: 82 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti Adhesion molecule antibody, anti CD31 antibody, anti CD31 antigen antibody, anti CD31 EndoCAM antibody, anti Endocam antibody, anti FLJ34100 antibody, anti FLJ58394 antibody, anti GPIIA antibody, anti GPIIA' antibody, anti PECA1 antibody, anti PECA1_HUMAN antibody, anti Pecam 1 antibody, anti PECAM 1 CD31 EndoCAM antibody, anti PECAM antibody, anti PECAM-1 antibody, anti Pecam1 antibody, anti Platelet endothelial cell adhesion molecule antibody, anti Platelet/endothelial cell adhesion molecule 1 antibody, anti Platelet/endothelial cell adhesion molecule antibody

IHC-P image of mouse kidney tissue using CD31 antibody (dilution of primary antibody at 1:500)

Immunohistochemical staining of paraffin embedded mouse skin tissue using anti-CD31 (primary antibody at 1:200)

IHC-P staining of mouse lung tissue using CD31 antibody (dilution at 1:200)

Immunohistochemical staining of paraffin embedded rat brain tissue using CD31 antibody (primary antibody at 1:100)

Immunohistochemical staining of mouse lung tissue using CD31 antibody (dilution of primary antibody - 1:200)

Western blot analysis of rat heart (lane 1), uterus (lane 2), ovary (lane 3), lung (lane4), trachea (lane 5) using CD31 antibody (primary antibody at 2 ug/ml)

Immunofluorescense analysis of mouse lung tissue using anti-CD31 (primary antibody at 1:200)

IHC-P staining of mouse lung tissue using CD31 antibody (dilution at 1:200)

IF analysis of mouse lung tissue using anti-CD31 (dilution of primary antibody at 1:200)

Immunohistochemical staining of paraffin embedded mouse skin tissue using CD31 antibody (primary antibody at 1:200)

Immunohistochemical staining of rat heart tissue using anti-CD31 (primary antibody at 1:100)

WB analysis of rat heart (lane 1), uterus (lane 2), ovary (lane 3), lung (lane4), trachea (lane 5) using CD31 antibody (dilution of primary antibody - 2 ug/ml)

IF analysis of rat kidney tissue using anti-CD31 (dilution of primary antibody at 1:100)

IHC-P image of rat skin tissue using CD31 antibody (dilution of primary antibody at 1:100)

IF analysis of rat lung tissue using anti-CD31 (dilution of primary antibody at 1:100)

IHC-P staining of rat lung tissue using anti-CD31 (dilution at 1:100)

IHC-P staining of mouse skin tissue using anti-CD31 (dilution at 1:100)

IHC-P staining of mouse kidney tissue using anti-CD31 (dilution at 1:100)

Immunofluorescence analysis of rat skin tissue using CD31 antibody (dilution of primary antibody - 1:100)

IHC-P staining of mouse skin tissue using CD31 antibody (dilution at 1:100)

Quick Database Links

Gene Symbol

CD31

UniProt

Q08481, Q3SWT0, P16284

NCBI Gene ID

5175

RefSeq:000433, 41, 000442

References

Hideo Kagaya 1, Alexander S Kim 1, Mian Chen 1, Pei-Yu Lin 1, Xuanzhi Yin 2, Matthew Spite 2, Michael S Conte Dynamic changes in proresolving lipid mediators and their receptors following acute vascular injury in male rats Physiol Rep, (2024)

PubMed: 39128880

Applications

ICC

Reactivity

Rat

Geórgia da Silva Feltran 1, Rodrigo Augusto da Silva 2, Célio Junior da Costa Fernandes 1, Marcel Rodrigues Ferreira 1, Sérgio Alexandre Alcântara Dos Santos 3, Luis Antônio Justulin Junior 3, Liliana Del Valle Sosa 4, Willian Fernando Zambuzzi Vascular smooth muscle cells exhibit elevated hypoxia-inducible Factor-1α expression in human blood vessel organoids, influencing osteogenic performance Exp Cell Res, (2024)

PubMed: 38909881

Applications

IF

Reactivity

Human

Nasrin Alborzi 1, Abdulbaset Maroofi 2, Zeinab Hafizi Barjin 3, Ali Moradi 4, Mohammad Ebrahim Rezvani 3, Fatemeh Safari Resveratrol attenuates pressure overload-induced myocardial remodeling in ovariectomized rats by rescuing the adaptive angiogenic response Life Sci, (2025)

PubMed: 40122333

Banafsheh Heidari 1, Negar Eidi 2, Pejman Mortazavi 2, Zahra Saffarian 3, Azadeh Soltani 4, Parvin Mansouri 5, Seyed Mehdi Tabaie Morphological characteristics, mitochondrial oxidoreductase activity, and vascularization of human adipose tissues pre-and post-xenotransplantation into BALB/c female nude mice Cryobiology, (2025)

PubMed: 40334370

S Behzadifard, M Navaei-Nigjeh, M Behzadifard Improving Pancreatic Islet Transplantation Using Fibrin Hydrogel Containing Microvascular Fragments in Subcutaneous Tissue of Type I Diabetic Rats researchsquare.com, (2025)

PubMed:

Majid Zamani a , Saeid Kaviani a , Mehdi Yousefi b , Saeid Abroun a , Mohammad Hojjat-Farsangi c , Behzad Pourabbas d Conditioned plasma promotes full-thickness skin defect healing in a rat model Regenerative Therapy, (2025)

PubMed:

Applications

IHC

Reactivity

Human

Jelodari, Sahar et al. Assessment of the Efficacy of an LL-37-Encapsulated Keratin Hydrogel for the Treatment of Full-Thickness Wounds ACS Appl Bio Mater, (2023)

PubMed: 37224450

Mai, Jingting et al. Tachycardia pacing induces myocardial neovascularization and mobilizes circulating endothelial progenitor cells partly via SDF-1 pathway in canines Heart Vessels, 31, 230-240 (2016)

PubMed: 25491934

Fernandez-Moure, Joseph S. et al. Cross-linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet-rich plasma in a rat model of hernia repair Wound Repair Regen, 25, 98-108 (2017)

PubMed: 28076885

Valipour, Elahe et al. Anti-angiogenic effects of testis-specific gene antigen 10 on primary endothelial cells Gene, 754, 144856 (2020)

PubMed: 32512160

Raisi, Abbas et al. Salvia miltiorrhiza hydroalcoholic extract inhibits postoperative peritoneal adhesions in rats BMC Complement Med Ther, 21, 126 (2021)

PubMed: 33879143