PDK4 Antibody

SKU: orb1939313

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:500-1:1000
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B567EV31X8X4
• Immunogen: Purified His-tagged PDK4 protein(Fragment) was used to produced this monoclonal antibody.
• Target: PDK4
• Molecular Weight: 46469 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4

All lanes: Anti-PDK4 Antibody at 1:250 dilution. Lane 1: K562 whole cell lysate. Lane 2: CEM whole cell lysate. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46469 Da. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PDK4 Antibody at 1:500-1:1000 dilution. Lane 1: K562 whole cell lysate. Lane 2: Human heart lysate. Lane 3: Mouse heart lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PDK4 Antibody at 1:1000 dilution + human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PDK4

UniProt

Q16654

RefSeq (Protein):NP_002603.1