PDK4 Antibody (Center D98)

SKU: orb1929615

Description

PDK4 Antibody (Center D98)

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 46469 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PDHK4

Confocal immunofluorescent analysis of PDK4 Antibody (Center D98) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 antibody (Center D98), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-PDK4 Antibody (Center D98) at 1:1000 dilution + HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q16654