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PDIA3/ERp57 Antibody
Description
Images & Validation
−| Tested Applications | IF, IHC, IHC-P, WB |
|---|---|
| Dilution Range | IF (1:50 - 1:500), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000) |
| Reactivity | Canine, Human, Monkey, Mouse, Rat |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Purified recombinant peptide derived from within residues 300 aa to the C-Terminus of human ERP57 produced in E. coli. |
| Target | PDIA3 / ERp57 |
| Purification | Immunoaffinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, 0.05% Sodium Azide, 20% Glycerol |
| Concentration | 2 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Anti-PDIA3 / ERp57 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence. Anti-ERP57 antibody in primary RPE cells at 1:100 dilution. Cells were fixed with 4% of PFA.

Western blot. Endogenous ERP57- endoplasmic reticulum lumen marker detected with PDIA3 / ERp57 antibody at 1:500 dilution. Lysates at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.
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PDIA3/ERp57 Antibody (orb1539440)
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