PDIA3/ERp57 Antibody

• Catalog Number: orb1539439
• Category: Antibodies
• Description: PDIA3/ERp57 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IF, IHC, IHC-P, WB
• Reactivity: Canine, Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: Purified recombinant peptide derived from within residues 300 aa to the C-Terminus of human ERP57 produced in E. coli.
• Concentration: 3 mg/ml
• Dilution range: IF (1:50 - 1:500), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000)
• Conjugation: Unconjugated
• Target: PDIA3 / ERp57
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.05% Sodium Azide, 20% Glycerol. PBS, 0.05% Sodium Azide, 20% Glycerol
• Alternative names: PDIA3, 58 kDa microsomal protein, ER protein 57, E
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-PDIA3 / ERp57 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence. Anti-ERP57 antibody in primary RPE cells at 1:100 dilution. Cells were fixed with 4% of PFA.

Western blot. Endogenous ERP57- endoplasmic reticulum lumen marker detected with PDIA3 / ERp57 antibody at 1:500 dilution. Lysates at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.