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PD-L2 Antibody / Programmed death ligand 2 / CD273

Catalog Number: orb607156

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb607156
CategoryAntibodies
DescriptionRecognizes a protein identified as PD-L2 (same as PDCD1LG2). Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. PD-L2 does not bind CD28, cytotoxic T lymphocyte A4 or ICOS (inducible co-stimulator). The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenA recombinant fragment of human PD-L2 protein within amino acids 27-220 was used as the immunogen for the PD-L2 antibody.
UniProt IDQ9BQ51
Tested applicationsELISA, FACS, IF, WB
Dilution rangeELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application notesOptimal dilution of the PD-L2 antibody should be determined by the researcher.
Antibody TypePrimary Antibody
Clone NumberPDL2/2676
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the PD-L2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Hazard InformationThis PD-L2 antibody is available for research use only.
NoteFor research use only
PD-L2 Antibody / Programmed death ligand 2 / CD273

Immunofluorescent staining of human Jurkat cells with PD-L2 antibody (green, clone PDL2/2676) and Reddot nuclear stain (red).

PD-L2 Antibody / Programmed death ligand 2 / CD273

Western blot testing of 1) human Raji, 2) human HepG2, 3) human ThP-1, 4) mouse RAW264.7, 5) mouse NIH-3T3 and 6) human U-87 MG cell lysate with PD-L2 antibody (clone PDL2/2676). Expected molecular weight: 31-60 kDa depending on glycosylation level.

PD-L2 Antibody / Programmed death ligand 2 / CD273

Flow cytometry testing of human Jurkat cells with PD-L2 antibody (clone PDL2/2676); Red=isotype control, Blue= PD-L2 antibody.

PD-L2 Antibody / Programmed death ligand 2 / CD273

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using PD-L2 antibody (clone PDL2/2676). These results demonstrate the foremost specificity of the PDL2/2676 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

PD-L2 Antibody / Programmed death ligand 2 / CD273

SDS-PAGE analysis of purified, BSA-free PD-L2 antibody (clone PDL2/2676) as confirmation of integrity and purity.

  • PD-L2 Antibody / Programmed death ligand 2 / CD273 [orb2642899]

    ELISA,  FACS,  IF,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • PD-L2 Antibody / Programmed death ligand 2 / CD273 [orb607123]

    FACS,  IF,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • PD-L2 Antibody / Programmed death ligand 2 / CD273 [orb2642797]

    FACS,  IF,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • PD-L2 Antibody / Programmed death ligand 2 / CD273 [orb640005]

    FACS

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • PD-L2 Antibody / Programmed death ligand 2 / CD273 [orb2643949]

    FACS

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg