PD-L2 Antibody / Programmed death ligand 2 / CD273

• Catalog Number: orb607156
• Category: Antibodies
• Description: Recognizes a protein identified as PD-L2 (same as PDCD1LG2). Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. PD-L2 does not bind CD28, cytotoxic T lymphocyte A4 or ICOS (inducible co-stimulator). The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PDL2/2676
• Tested applications: ELISA, FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant fragment of human PD-L2 protein within amino acids 27-220 was used as the immunogen for the PD-L2 antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This PD-L2 antibody is available for research use only.
• UniProt ID: Q9BQ51
• Storage: Store the PD-L2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PD-L2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.