PD-L1/CD274 Intracellular domain (IHC prefer) Antibody

SKU: orb1294268

Description

Rabbit polyclonal antibody to PD L1

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Dilution range: Western Blot & Immunoprecipitation: Reported in the literature, not verified in house. Immunofluorescence 1:100-1:300 Immunohistochemistry (Paraffin) 1:100-1:250
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human PD-L1 / CD274
• Target: PD-L1 / CD274 Intracellular domain (IHC prefer)

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. stained with orb1294268 anti-PD-L1 / CD274 antibody (red) at 1:200 and 4°C.

Cell: MDA-MB-231 1Ab: orb1294268 1-500 2Ab: anti Rabbit IgG (Light chain) 1-3000.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1 / CD274 with orb1294268 at 1/150.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1 / CD274 with orb1294268 at 1/150.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PD-L1 / CD274 with orb1294268 at 1/150.

Quick Database Links

Gene Symbol

PD-L1 / CD274 Intracellular domain (IHC prefer)