CD274 Rabbit Polyclonal Antibody

SKU: orb629783

Description

Rabbit polyclonal antibody to CD274

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, IP, WB
• Dilution Range: WB: 1:200-1:1000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: CD274 molecule
• Target: CD274
• Molecular Weight: 50 kDa, 70 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1, PD-L1/CD274, Programmed death ligand 1

mouse heart tissue were subjected to SDS PAGE followed by western blot with orb629783 (PD-L1/CD274 Antibody) at dilution of 1:300.

Immunofluorescent analysis of HEK-293 cells using orb629783 (PD-L1/CD274 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-PD-L1/CD274 (IP: orb629783, 4 ug; Detection: orb629783 1:1000) with mouse heart tissue lysate 4000 ug.

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using orb629783 (PD-L1/CD274 Antibody) at dilution of 1:50.

Quick Database Links

Gene Symbol

CD274

UniProt

Q9NZQ7