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Catalog Number | orb629783 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CD274 |
Target | CD274 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | liquid |
Purity | ≥95% as determined by SDS-PAGE |
Purification | Immunogen affinity purified |
Immunogen | CD274 molecule |
UniProt ID | Q9NZQ7 |
MW | 50 kDa, 70 kDa |
Tested applications | ELISA, FC, IF, IHC, IP, WB |
Dilution range | WB: 1:200-1:1000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 mole Read more... |
Note | For research use only |
mouse heart tissue were subjected to SDS PAGE followed by western blot with orb629783 (PD-L1/CD274 Antibody) at dilution of 1:300.
Immunofluorescent analysis of HEK-293 cells using orb629783 (PD-L1/CD274 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
IP Result of anti-PD-L1/CD274 (IP: orb629783, 4 ug; Detection: orb629783 1:1000) with mouse heart tissue lysate 4000 ug.
Immunohistochemistry of paraffin-embedded mouse heart tissue slide using orb629783 (PD-L1/CD274 Antibody) at dilution of 1:50.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |