CD274 rabbit pAb

SKU: orb771666

Description

Rabbit polyclonal antibody to CD274

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution range: IHC-p 1:50-200, ELISA 1:10000-20000 IF 1:100-300 Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide from human protein at AA range: 181-230
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274) antibody

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100.