PAX7 Antibody

SKU: orb749730

Description

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

Research Area

Cancer, Developmental Biology

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the PAX7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: PAX7/1187
• Immunogen: Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of formalin-fixed, paraffin-embedded human Rhabdomyosarcoma with PAX7 antibody (clone PAX7/1187). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH9, for 10-20 min.

IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with PAX7 antibody (clone PAX7/1187). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH9, for 10-20 min.

SDS-PAGE analysis of purified, BSA-free PAX7 antibody (clone PAX7/1187) as confirmation of integrity and purity.

Quick Database Links

UniProt

P23759