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Catalog Number | orb1938950 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Target | This PAX7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-437 amino acids from the C-terminal region of human PAX7. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
UniProt ID | P23759 |
MW | 55119 Da |
Tested applications | IHC-P, WB |
Dilution range | WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100 |
Antibody Type | Primary Antibody |
Clone Number | RB28560 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Paired box protein Pax-7, HuP1, PAX7, HUP1 |
Note | For research use only |
NCBI | NP_002575.1, NP_039236.1, NP_001128726.1 |
Anti-PAX7 Antibody (C-term) at 1:1000 dilution + Mouse skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-PAX7 Antibody (C-term) at 1:2000 dilution + C2C12 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
PAX7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAX7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.