PAX4 Antibody

SKU: orb1538071

Description

PAX4 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
• Reactivity: Human, Rat
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide located between aa166-215 of human PAX4 (A4D0Z1, NP_006184). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey (100%); Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (92%).
• Target: PAX4
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.09% sodium azide, 2% sucrose
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PAX4, KPD, Paired box protein Pax-4, Paired domain gene 4, Paired box 4, Paired box gene 4, MODY9

Quick Database Links

Gene Symbol

PAX4