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Item 1 of 4
PAX4 Antibody (Center)
Catalog Number: orb1929883
| Catalog Number | orb1929883 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB20216 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human, Rat |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 37833 Da |
| Target | This PAX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human PAX4. |
| UniProt ID | O43316 |
| NCBI | NP_006184.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Paired box protein Pax-4, PAX4 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of widr cells using PAX4 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with PAX4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PAX4 antibody (Center) in CEM cell line lysates (35 ug/lane). PAX4 (arrow) was detected using the purified Pab.
All lanes: Anti-PAX4 Antibody (Center) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: rat liver lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
