PAX4 Antibody

SKU: orb1249188

Description

PAX4 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: EIA, ELISA, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Pancreas lysates and in lysates of cell lines A431, HeLa and K562 (calculated MW of 37.1kDa according to Human NP_006184.2 and 38.0kDa according to Mouse NP_035168.1). Recommended concentration: 0.2-0.6µg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAX4 captured by a rabbit antibody. Recommended reporter concentration: 1-2µg/mL

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GKLATATSLPEDTVR
• Target: PAX4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PAX4, paired box 4, KPD, MGC129960, MODY9, paired box gene 4, paired domain gene 4

Quick Database Links

Gene Symbol

PAX4

UniProt

O43316

RefSeq (Protein):NP_006184.2