PAX4 Antibody

• Catalog Number: orb1249188
• Category: Antibodies
• Description: PAX4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, WB
• Predicted Reactivity: Canine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-GKLATATSLPEDTVR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Pancreas lysates and in lysates of cell lines A431, HeLa and K562 (calculated MW of 37.1kDa according to Human NP_006184.2 and 38.0kDa according to Mouse NP_035168.1). Recommended concentration: 0.2-0.6ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAX4 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PAX4
• UniProt ID: O43316
• NCBI: NP_006184.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PAX4, paired box 4, KPD, MGC129960, MODY9, paired
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Pancreas lysates and in lysates of cell lines A431, HeLa and K562 (calculated MW of 37.1kDa according to Human NP_006184.2 and 38.0kDa according to Mouse NP_035168.1). Recommended concentration: 0.2-0.6ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAX4 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
• Expiration Date: 12 months from date of receipt.

orb1249188 (0.2 ug/ml) staining of Mouse Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249188 (1.5 ug/ml) as the reporter antibody.