PARP2 Antibody

• Catalog Number: orb1538016
• Category: Antibodies
• Description: PARP2 Antibody
• Target: PARP2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-LDLFEVEKDGEKE, from the internal region of the protein sequence according to NP_005475.1.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:8000), WB (1 - 3 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B3239 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3239 was determined to be 2.5 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PARP2, ADPRT2, ADPRTL2, ADPRTL3, ADPRT-2, ARTD2, H
• Note: For research use only

Antibody (1 ug/ml) staining of Human Spleen Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.