PARP2 Antibody

SKU: orb1247144

Description

PARP2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in Human Spleen lysates (calculated MW of 60.8kDa according to NP_005475.1. Recommended concentration: 1-3µg/mL.Immunohistochemistry:Paraffin embedded Human Skin and Adrenal Gland. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-LDLFEVEKDGEKE
• Target: PARP2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PARP2, poly (ADP-ribose) polymerase family, member 2, HGNC:272, ADPRT2, ADPRTL2, ADPRTL3, PARP-2, pADPRT-2 , ADP-ribosyltransferase (NAD poly ADP-ribose polymerase)-like 2, poly (ADP-ribosyl) transferase-like 2, poly(ADP-ribose) synthetase

orb1247144 (1 ug/ml) staining of Human Spleen Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247144 (5 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247144 (5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

PARP2

UniProt

Q9UGN5

RefSeq (Protein):NP_005475.1