PARP12 Antibody

SKU: orb1248618

Description

PARP12 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0.03µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunofluorescence: Anonymous customer has confirmed specificity by siRNA-mediated PARP2 knockdown.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NAHDIKNKSSAP
• Target: PARP12
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PARP12, poly (ADP-ribose) polymerase family, member 12, HGNC:21919, FLJ22693, PARP-12, ZC3H1, ZC3HDC1, zinc finger CCCH type domain containing 1, zinc finger CCCH-type domain containing 1

Quick Database Links

Gene Symbol

PARP12

UniProt

Q9H0J9

RefSeq (Protein):NP_073587.1